Ursolic acid, anethole and β-sitosterol may be the main active components of DMSCO in the treatment of AD.

Three key compounds, namely ursolic acid, anethole and β-sitosterol were obtained from the analysis of the key targets. These key targets were involved in a variety of biological processes, including amyloid deposition, apoptosis, autophagy, inflammatory response and oxidative stress and pathways, such as the PI3K-AKT, MAPK and TNF pathways. A total of 43 key targets for the treatment of AD were obtained from the topological analysis of the DMSCO-AD target network. A total of 172 AD-related genes and 37 shared targets of DMSCO and AD were identified. ResultsĪ total of 30 DMSCO effective compounds and 209 effective drug targets were obtained. Subsequently, gene enrichment in Gene Ontology (GO) and KEGG pathways were conducted using the David 6.8 online tool. The network interaction model of the compound-target-disease was established and was used to obtain the key targets of DMSCO on AD through network topology analysis. The AD-related target proteins were identified from the OMIM, DisGeNet, GEO and GeneCards databases. The active components of DMSCO were collected from the TCMSP and ETCM databases and the target proteins of these compounds were predicted using TCMSP, SwissTargetPrediction and the STITCH database. The present study aimed to investigate the active ingredients and effective mechanisms of DMSCO for the treatment of AD based on a network pharmacology approach.

et Zucc., DMSCO) is a Chinese herb that can be used for the treatment of Alzheimer’s disease (AD), but its mechanism remains unknown. Shanzhuyu (the dried mature sarcocarp of Cornus officinalis Sieb.

Cytoscape 3 increase memory